Journal: Oncotarget

Article Title: KIAA1114, a full-length protein encoded by the trophinin gene, is a novel surface marker for isolating tumor-initiating cells of multiple hepatocellular carcinoma subtypes

doi:

Figure Lengend Snippet: ( A and B ) Flow cytometric analysis of surface KIAA1114 expression in human CC cell lines (A) and AFP + and AFP − HCC cell lines (B) . (C) Immunoblot analysis of tissue lysates from normal liver (N), patient tumor (PT), and its adjacent normal tissue from the same donor (PN). β-actin served as an internal control.

Article Snippet: Human liver whole tissue lysate and paired primary tumor and normal tissues were purchased from Novus Biologicals.

Techniques: Expressing, Western Blot, Control

Journal: Journal of Clinical and Translational Hepatology

Article Title: Preclinical Long-term Magnetic Resonance Imaging Study of Silymarin Liver-protective Effects

doi: 10.14218/JCTH.2021.00499

Figure Lengend Snippet: (A) Prussian blue staining evaluated iron accumulation. (B) Iron accumulation significantly decreased in the TAA-SY group compared with the TAA group. (C) Western blot assays of ferritin and hepcidin protein expression. (D) Ferritin in the TAA-SY group was decreased compared with the TAA group. Hepcidin in the TAA-SY group was slightly increased compared with the TAA group. (E) T 2 * values and iron overload (%) were negatively correlated (* p <0.05, ** p <0.01, *** p <0.001). Con, control; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; TAA, thioacetamide-injected; TAA-SY, TAA-injected and silymarin-treated.

Article Snippet: Western blots reacted with primary antibodies against ferritin (3998S, Cell Signaling Technology, Beverly, MA, USA), hepcidin (M01347, Boster Biological Technology, Pleasanton, CA, USA) and GAPDH (ab8245, Abcam, USA), followed by HRP-conjugated secondary antibodies.

Techniques: Staining, Western Blot, Expressing, Control, Injection